Hopefully, CRISPR-based diagnostics will make an early debut amid COVID-19 outbreak. But what about a CRISPR prophylactic strategy to combat coronaviruses? The proof of concept is here, in bioRxiv, but it will be deployed in the next pandemic if we are lucky. It’s called PAC-MAN, like the videogame, stands for Prophylactic Antiviral CRISPR in huMAN cells, and comes from the Stanley Qi Lab.Continue reading
Do you remember prime editing? It’s the new ‘search-and-replace’ genome editing technology that mediates targeted insertions, deletions, all 12 possible base-to-base conversions, and combinations thereof. The first good news is that David Liu et al. adapted prime editors for use in rice and wheat, so don’t miss their paper in Nature Biotechnology.Continue reading
The DNA double helix as a metaphor for the relationship between genetics and SciFi – novels and movies on the one strand and scientific breakthroughs on the other strand. It’s courtesy of graduated student Kartik Lakshmi Rallapalli, who examines the science and fiction timelines in a post for the Addgene blog.Continue reading
From Meganucleases to Prime Editing, download the high resolution cards!
Do you remember Sherlock? The CRISPR-based platform was heralded in Science as a new generation of low cost diagnostic tests with single-base specificity, easy to use even when oubreaks occur in remote areas. The good news is that Feng Zhang and colleagues are sharing a research protocol, applicable to purified RNA, that may inform the development of a Sherlock test for COVID-19. For more information, visit the McGovern Institute website.