The US Department of Agriculture has given the green light to five CRISPR-edited plants in the last couple of years. See the table below, published by Nature Biotechnology this month. CRISPR is set to make its commercial debut in fields in 2020, with DuPont Pioneer’s waxy corn, and hopes are high that gene editing will give us the chance of rewriting the GMO debate. Continue reading
2017 ends with over 3,000 CRISPR papers indexed by PubMed and 6,810,000 Google’s search results. There is no doubt that 2018 is going to be hot, with the brand new CRISPR Journal launching in 2018 and leading scientists convening at a super CRISPR meeting in Lithuania next June. Clinical trials for genetic diseases such as beta-thalassemia and Leber congenital amaurosis are expected to start, and a forthcoming European Court verdict could be a turning point for the future of edited crops. Happy new year and happy new edit then, surprisingly surprising surprises ahead!
The first patient edited “in vivo” last week is a breaking news story, and zinc finger nuclease ZFN must be credited for the accomplishment. A putatively outdated system stealing the scene from the most celebrated technique for gene editing is a bit like Carl Lewis beating Usain Bolt at the Rio Olympics. Any wonder that tweets by some biotech-enthusiasts had something of a derby atmosphere, while many inattentive readers thought it was CRISPR stuff, as lay people never heard of ZFN before. Continue reading
So far we have learned that CRISPR may turn a faulty gene off by cutting and mutating its sequence. But what if we want to proceed more cautiously and avoid permanent changes to the genome? We could leave the target gene intact but ineffective, by intercepting and destroying the RNA messages with which it gives the wrong orders to the diseased cells. In this way it would be easier to go back if necessary. The good news is that CRISPR is a jack-of-all-trades, well-suited for the task, and the new approach (call it RNA targeting with CRISPR) is going to help to study human biology and diseases. One of the technique pioneer, Feng Zhang, has demonstrated in Nature last week that it can efficiently target RNA in mammalian cells (and also plants), equalizing and even surpassing the performance of the current tool of choice for RNA knockdown (RNA interference). In short, besides advancing its career as DNA editor, CRISPR has also found a second job in the RNA business. Continue reading
They are the first human embryos edited in Europe and reported in scientific literature. The key difference with experiments already carried out in China and US is that the research published by Nature last week doesn’t have embryonic gene therapy in view. The London Francis Crick’s Institute team, in fact, was not interested in correcting disease-causing mutations but in increasing knowledge on human embryonic development. We asked one of the authors, Alessandro Bertero, to explain goals and results. The Italian researcher was pursuing his Ph.D. at Cambridge when he helped to refine the technique used by Kathy Niakan and colleagues to edit the genome of embryos. He answered our questions via Skype from America, where he continues working on embryonic stem cells as a postdoctoral fellow at Washington University Continue reading
Biodiversity is a wonderful interplay between genetics and evolution, and butterflies are a fascinating example with their variety of patterns and colors. Understanding how the same gene networks engender visual effects so diverse in thousands of Lepidoptera species is a longtime ambition for many entomologists and evolutionary biologists. The good news is that scientists nowadays have a straightforward technique working with organisms that were difficult to manipulate with conventional biotech tools. Obviously, we are talking about CRISPR. Two papers published in PNAS last week describe how genome editing was used to alter the genetic palette of colors in butterflies and how their wings changed as a result. We’ve asked the entomologist Alessio Vovlas, from the Polyxena association, to comment these stunning experiments. Continue reading