Playing with Chromosomes: CRISPR’s New Frontier

Humans have 23 pairs of chromosomes. The record-holder among animals (a butterfly called Polyommatus atlantica) can boast 229. Some plants have even more, but only because their genomes have undergone multiple rounds of duplication. We’re talking about chromosomes, of course. Their number is characteristic of each species and still shrouded in mystery. Why that number? And what would happen if we changed it?
In animals, the effects tend to be detrimental: mice with fused chromosomes, for instance, show abnormalities in behavior, growth, and fertility. Plants, however, appear surprisingly flexible, as demonstrated by a new experiment using CRISPR, recently published in Science.

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CRISPR Wow: Arabidopsis Dresses Up

For scientists, it is a model organism—perfect for research because it grows fast, self-pollinates, and has a small genome that is easy to modify. In fact, it is the most studied plant in the world, the first to have its DNA sequenced, a true diva of genetics. To everyone else, it is an anonymous weed: among countless botanical wonders, Arabidopsis thaliana hardly stands out, being so small and with such unremarkable flowers.
But the foundational knowledge is there, and so are the biotechnological tools. So, why not give its colorless, modest bloom a gala makeover, with petals to make a rose jealous? This idea sparked the imagination of Nick Desnoyer from the University of Zurich. With a little breeding and a touch of CRISPR, he created a plant with the genetic backbone of Arabidopsis but adorned with a sumptuous corolla.
Don’t miss his visually stunning thread on X and bluesky where he announces a bio-art project set to blossom in 2025.

Plant editing gets easier with CRISPR loaded pollen

maisPollination is a natural way to deliver DNA into plant cells. So why not to use pollen as a vehicle for CRISPR machinery to start genome editing? HI Edit, as this approach is called, has been successfully tested by Syngenta in corn, Arabidopsis and wheat in the lab. Please see the paper just published in Nature Biotechnology by Timothy Kelliher et al., Jon Cohen’s piece in Science, and a quick guide to HI (haploid induction) from Current Biology. Leading wheat geneticist Cristobal Uauy of the John Innes Centre also showed high spirit, when asked for comment by email: “The possibility to genome edit any variety is revolutionary given that transformation is so difficult in many species. If I understand this correctly this would be a game changer as it would allow us to alter genes in elite cultivars.”